FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l02n01 handrdnfgfrercc2.34100000034e60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l02n01 handrdnfgfrercc2.34100000034e60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19328415
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT313690.16229473549693546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGTGG120000.014.6049063
GAGTACT99300.014.45531220
AGCAGTG129150.013.7097882
AGTGGTA134100.013.0357145
GTGGTAT135150.012.9843786
TACGGGG32100.012.4056922
GTACGGG50000.012.32921621
AGAGTAC141800.012.01090419
AGTACGG52200.011.93892820
CGCAGAG144650.011.92951716
CAGTGGT152200.011.7219844
AGTACTT124850.011.67727221
GGTATCA153800.011.5854118
TGGTATC154700.011.4743817
GAGTACG54300.011.39433419
ACGCAGA158800.011.079041515
GTACTTT134250.010.94338222
CAGAGTA160150.010.84522318
GTATCAA164100.010.8171049
GCAGAGT161500.010.740581517
Produced by FastQC (version 0.11.2)