FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l02n01 handrdnfgfr15hpfercc3.34100000034f1f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l02n01 handrdnfgfr15hpfercc3.34100000034f1f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18969086
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT275000.1449727203514181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT82800.015.27082920
GCAGTGG108050.013.8466393
GTACGGG42850.013.65146221
AGTACGG43350.013.49400520
AGTGGTA113200.013.2961885
AGCAGTG116550.012.9915382
GTGGTAT116600.012.9084796
AGAGTAC114350.012.59216719
GAGTACG47750.012.48616819
CGCAGAG118150.012.33931416
TACGGGG27300.012.27942322
AGTACTT106100.012.06572721
GGTATCA132650.011.3296568
GTACTTT114800.011.19047622
GCAGAGT132700.011.13894917
CAGTGGT135100.011.1075434
TGGTATC134450.011.0775747
CAGAGTA133400.011.06369218
ACGCAGA134200.010.98091615
TCAACGC136700.010.73066312
Produced by FastQC (version 0.11.2)