FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l02n01 handrdnfgfr15hpfercc1.34100000034ddb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l02n01 handrdnfgfr15hpfercc1.34100000034ddb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19049385
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT306450.16087133521633373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGTGG120200.015.0111333
GAGTACT101850.014.64572620
AGCAGTG131050.013.7855592
AGTGGTA131500.013.6356595
GTGGTAT137650.013.0427836
AGAGTAC137900.012.611719
CAGTGGT148550.012.2220624
CGCAGAG144450.012.195216
AGTACTT124200.012.13700821
GGTATCA150600.011.9809988
TGGTATC154850.011.72481257
CAGAGTA157350.011.20987718
ACGCAGA158750.011.11084515
TCAACGC160100.011.1014412
GCAGAGT159100.011.08640117
GTATCAA163000.011.0557579
GTACTTT137600.011.02043522
ATCAACG164850.010.958981511
CAACGCA164700.010.95530213
AACGCAG165350.010.62650314
Produced by FastQC (version 0.11.2)