FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l02n01 foxfcamras15hpfercc3.34100000034f53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l02n01 foxfcamras15hpfercc3.34100000034f53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23790438
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT335760.14113233224205457No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261950.11010726242198651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT90300.016.96765120
AGAGTAC121050.014.31160419
CGCAGAG123600.014.12520116
AGTACGG43550.013.53553720
GCAGTGG135500.013.4158723
GTACGGG43250.013.26519621
AGTGGTA136150.013.2526745
GTGGTAT136450.013.0916336
AGTACTT119050.013.002254521
GAGTACG46000.012.76571119
ACGCAGA140450.012.59076815
AGCAGTG144400.012.4489772
GCAGAGT144250.012.44624817
CAGAGTA142900.012.31202718
GTACTTT132100.011.97320222
GGTATCA152600.011.8388048
TGGTATC154200.011.7889147
CAACGCA153450.011.64127113
TACGGGG29650.011.60979422
ATCAACG155550.011.44071911
Produced by FastQC (version 0.11.2)