FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l02n01 foxfcamras15hpfercc2.34100000034ea4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l02n01 foxfcamras15hpfercc2.34100000034ea4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20760695
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT310130.14938324559943683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT96850.015.30878120
GCAGTGG124400.014.5946383
AGCAGTG131450.013.7436952
AGTGGTA135500.013.3160465
GTGGTAT138050.012.9886256
AGAGTAC140650.012.57296419
CGCAGAG142800.012.35179616
AGTACTT123450.012.26531821
GGTATCA150000.012.0438558
TGGTATC158450.011.5577537
CAGTGGT160750.011.3783624
GTACTTT134550.011.30357322
CAGAGTA158950.011.16762518
TCAACGC157950.011.16702912
ACGCAGA160200.011.12256515
GCAGAGT161950.010.90513417
GTATCAA164450.010.8898129
ATCAACG169400.010.58488611
CAACGCA169400.010.50517513
AACGCAG169750.010.47026114
Produced by FastQC (version 0.11.2)