FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l02n01 foxfcamras15hpfercc1.34100000034e03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l02n01 foxfcamras15hpfercc1.34100000034e03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21001695
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT302240.14391219375388511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT95850.014.95230120
GCAGTGG125500.013.9830283
AGCAGTG132100.013.3525952
AGTGGTA136350.012.9198355
GTGGTAT137200.012.7742916
AGAGTAC136700.012.54143319
CGCAGAG140500.012.33013616
AGTACTT121800.012.06217421
GGTATCA147900.011.8805448
TGGTATC152600.011.6030917
ACGCAGA156650.011.11639715
CAGAGTA156150.011.09443918
TCAACGC158850.011.0474212
GTACTTT135000.010.96603922
GCAGAGT158050.010.93251917
CAGTGGT162000.010.9019724
GTATCAA161650.010.8838999
AACGCAG166700.010.62161414
CAACGCA167800.010.48492113
GTACGGG51600.010.46456621
Produced by FastQC (version 0.11.2)