FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l08n01_sv110.351000000409f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l08n01_sv110.351000000409f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21141657
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC1934180.914866795918598TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT252200.038.55936442
TCGTATG253300.038.4807243
CGTATGC253250.038.43502444
TCTCGTA254700.038.1985541
GTATGCC257700.037.8411645
ATCTCGT259700.037.35050640
ACGGCTA262300.036.97160332
CATCTCG259750.036.780439
CGGCTAC263600.036.43353333
TCACGGC277500.035.1043530
AGTCACG286400.034.6501928
ATCGGAA307350.034.6132052
GATCGGA304000.034.5740971
GTCACGG285700.034.47504429
CACGGCT286150.034.08252331
CTACATC278700.033.9246436
CGTCTGA310700.033.32703416
TCGGAAG321700.033.0762183
GCTACAT288500.033.05335635
ACACGTC316200.032.911213
Produced by FastQC (version 0.11.2)