FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l08n01_sv109.3510000004097c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l08n01_sv109.3510000004097c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39939085
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC2738580.6856892189693379TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA361300.039.775711
TATCTCG331600.039.45446839
ATCGGAA372900.039.1307952
CTCGTAT347600.039.01034542
TCTCGTA349750.038.8219941
TCGTATG351050.038.652643
CGTATGC354200.038.32790444
CGTCTGA377500.037.86956816
ATCTCGT359650.037.73458540
ACACGTC381050.037.7178613
ACGTCTG382150.037.47352215
TCGGAAG389700.037.357253
CGGAAGA395950.036.684574
GTATGCC371850.036.59334645
GCTTATC358100.036.45333536
CACGTCT399100.035.9158214
TAGCTTA374950.034.9835334
CACACGT411850.034.94840612
GTCACTA384550.034.49922629
ACTAGCT385700.034.27507432
Produced by FastQC (version 0.11.2)