FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l08n01_sv108.35100000040902.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l08n01_sv108.35100000040902.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59237307
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC34166915.767802712570981TruSeq Adapter, Index 9 (100% over 51bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC922940.15580384165674513TruSeq Adapter, Index 9 (100% over 46bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3942150.044.4501461
ATCGGAA3988800.044.372942
TCGGAAG3990300.044.0478063
CGGAAGA3966750.043.9866564
GTATGCC3873200.042.7444145
ACACGTC4112800.041.87650313
GCACACG4150650.041.60978711
CGTCTGA4103550.041.583316
CACACGT4148550.041.5757612
ACGTCTG4123600.041.541515
CACGTCT4142650.041.5133814
CGTATGC4074900.040.6192644
ACGATCA4053400.040.59863332
GTCACGA4090500.040.5829729
CGATCAG4052400.040.57964733
TCTCGTA4076700.040.56987441
CTCGTAT4079050.040.565842
GATCTCG4072350.040.56238639
CACGATC4066700.040.5553331
GAAGAGC4306400.040.5520446
Produced by FastQC (version 0.11.2)