FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l08n01_sv107.35100000040897.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l08n01_sv107.35100000040897.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60891637
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC13881102.2796398132636835TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1610500.043.6476671
ATCGGAA1636350.043.4462742
TCGGAAG1646250.043.1111953
CGGAAGA1649550.042.9225734
CGTCTGA1635600.042.76864216
ACGTCTG1643450.042.60952815
ACACGTC1648750.042.5862913
AATCTCG1576850.042.1401739
TCTCGTA1596200.041.9464241
CTCGTAT1596650.041.91346442
TCGTATG1608550.041.6299643
CACGTCT1687450.041.5958114
CACACGT1689550.041.59432612
CGTATGC1608200.041.58446544
GTATGCC1609850.041.55861345
ATCTCGT1610800.041.54946540
GCACACG1721950.040.8902511
GTCACAC1698550.039.95564329
TTGAATC1673500.039.8020836
GAACTCC1755050.039.62568321
Produced by FastQC (version 0.11.2)