FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l08n01_sv106.35100000040810.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l08n01_sv106.35100000040810.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42745488
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC1991580.465915841222821TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT250700.039.18520742
TCTCGTA252250.038.89983741
TCGTATG253200.038.82496643
CGTATGC257200.038.21240244
ATCTCGT264950.037.11164540
ATCGGAA286050.036.7747572
GATCGGA282300.036.7601361
CATCTCG271000.035.92619739
CGTCTGA290700.035.44217316
ACACGTC292400.035.36727513
GTATGCC282950.034.83822345
ACGTCTG301900.034.19439315
TCGGAAG310050.033.9136243
CACGTCT315150.032.8352614
CGGAAGA320450.032.711384
CACACGT327100.031.7272412
TAGGCAT306000.031.62637335
TTAGGCA316100.030.79376834
TCACTTA331250.029.9769930
GCACACG348400.029.97480611
Produced by FastQC (version 0.11.2)