FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l08n01_sv103.351000000407db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l08n01_sv103.351000000407db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36486604
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC11939093.272184498179113TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1347250.043.8341941
ATCGGAA1366500.043.7418142
CGTCTGA1358550.043.63492616
TCGGAAG1372150.043.5600623
ACGTCTG1362350.043.5429415
ACACGTC1365050.043.51508313
CGGAAGA1373500.043.426014
TCTCGTA1340100.043.1825141
CTCGTAT1340050.043.14047642
TCGTATG1341600.043.0872843
CGTATGC1342400.043.05322344
CATCTCG1337900.043.0332639
ATCTCGT1353850.042.81040240
CACGTCT1388800.042.7849214
GTATGCC1354200.042.6880445
CACACGT1394200.042.68338812
GCACACG1405000.042.4225411
GATCATC1363900.042.04673836
AGTCACC1379000.041.82929228
GTCACCA1383400.041.6588229
Produced by FastQC (version 0.11.2)