FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l07n01_sv105.35100000040ae5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l07n01_sv105.35100000040ae5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39042983
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC6508151.6669192515336237TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA749450.043.1482051
ATCGGAA751350.042.997312
TCGGAAG760100.042.333563
CGTCTGA744700.042.11586816
CGGAAGA762750.042.0612874
ACACGTC750100.041.99695613
CGATCTC721700.041.9272738
ACGTCTG751450.041.79144315
ACGATCT725200.041.7218137
GATCTCG727600.041.71713639
CACGATC724550.041.65988536
TCTCGTA729000.041.65862341
CTCGTAT729200.041.65645242
TCACGAT728950.041.46088835
TCGTATG734150.041.36332743
ATCACGA733050.041.26582334
CATCACG735350.041.25996433
CGTATGC735800.041.25222844
ATCTCGT738850.041.07584840
CACGTCT769550.040.9488114
Produced by FastQC (version 0.11.2)