FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l07n01_sv104.35100000040a88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l07n01_sv104.35100000040a88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31954592
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC13589524.252759665966006TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1480250.044.5107151
ATCGGAA1482750.044.3683932
TCGGAAG1492750.044.0003053
CGGAAGA1498500.043.7175254
ACACGTC1478600.043.63407513
CGTCTGA1478550.043.5596416
ACGTCTG1480900.043.51937515
CACGTCT1493000.043.20735514
CACACGT1499950.043.11493712
GCACACG1502750.043.08687211
TCTCGTA1478050.042.95319741
AATCTCG1476100.042.93526539
CTCGTAT1479000.042.92104742
TCGTATG1480850.042.8826243
CGTATGC1482300.042.83308444
ATCTCGT1487050.042.64936440
GTATGCC1496600.042.44936445
GTAATCT1505700.042.00904537
GAACTCC1550100.041.3416421
TAATCTC1534000.041.29123738
Produced by FastQC (version 0.11.2)