FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l07n01_bgm17.35100000040a1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l07n01_bgm17.35100000040a1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36959754
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC5068771.3714295825670269TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA556150.043.980751
ATCGGAA561100.043.5355262
CGCCAAT546500.043.06620433
CGTCTGA561100.042.91534816
TCGGAAG568500.042.909463
ACACGTC562800.042.84283413
ACGCCAA551500.042.70530732
GTCACGC555950.042.63673829
TCTCGTA555050.042.59661541
ACGTCTG566050.042.5758315
CTCGTAT555350.042.54929742
CGGAAGA573800.042.4761624
TCGTATG558000.042.40366743
TATCTCG559250.042.3410739
CGTATGC558900.042.3031844
AGTCACG562800.042.12579328
ATCTCGT562800.042.06240
TCACGCC563600.041.89426830
CACGCCA565000.041.72273331
CACGTCT579350.041.59842714