FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l07n01_bgm16.351000000409a3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l07n01_bgm16.351000000409a3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33293070
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC21997346.607182816123595TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2363600.044.6239241
ATCGGAA2365000.044.5562322
TCGGAAG2367200.044.4416243
CGGAAGA2370900.044.2701954
ACACGTC2352900.044.20723313
CGTCTGA2349400.044.17108516
ACGTCTG2355250.044.12536615
CACGTCT2364900.043.96338714
GCACACG2371650.043.96332611
CACACGT2373750.043.8782512
TCTCGTA2354500.043.45302641
CTCGTAT2355500.043.44603742
TCGTATG2358200.043.4191943
CGTATGC2359450.043.38760844
GATCTCG2356500.043.37796439
GTATGCC2363050.043.341545
ATCTCGT2359350.043.3188940
GAACTCC2414150.042.7818221
AGTGATC2380500.042.74879536
GTGATCT2392750.042.55626337
Produced by FastQC (version 0.11.2)