FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l07n01_bgm15.3510000004091f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l07n01_bgm15.3510000004091f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31948036
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC5304421.6603274141797009TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA592550.044.1880071
ATCGGAA597300.043.763832
TCGGAAG604800.043.142983
ACACGTC591000.043.1123413
CGTCTGA591950.043.01906616
ACGTCTG595600.042.7629915
CGGAAGA609200.042.7333264
TCTCGTA591000.042.1021741
TCGTATG590750.042.0971443
CTCGTAT590650.042.0966542
CGTATGC592100.041.95176744
GCACACG611200.041.937911
AATCTCG594600.041.9115839
CACGTCT608450.041.86356714
CACACGT614350.041.6678112
ATCTCGT597250.041.6239240
GTATGCC614200.040.48255545
ACCAATC633500.038.98290336
GACCAAT636950.038.81413735
ACTGACC643650.038.6761532
Produced by FastQC (version 0.11.2)