FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l07n01_bgm14.351000000408a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l07n01_bgm14.351000000408a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52648931
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC4025530.7645986202454899TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA459800.042.7132641
ATCGGAA465100.042.218762
CGATGTA446400.042.17439734
CACCGAT450600.042.02614231
ACCGATG451050.041.9383232
CTCGTAT451550.041.882742
TCTCGTA452350.041.8583641
TCGTATG453900.041.7203843
CGTCTGA462400.041.664116
TATCTCG454150.041.58348539
ACACGTC465000.041.5291213
CGTATGC456300.041.4861544
CCGATGT455400.041.42567433
GTCACCG461400.041.14000329
ACGTCTG470400.041.00335315
TCGGAAG480500.040.828183
ATCTCGT466150.040.69156640
TCACCGA467800.040.5049930
CGGAAGA486300.040.2713134
CACGTCT486850.039.705714
Produced by FastQC (version 0.11.2)