FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l06n01_glk1_chx_pn_pd_01i.3510000004088a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l06n01_glk1_chx_pn_pd_01i.3510000004088a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23550835
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC3292411.3980013872119608TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG366700.043.3109411
CACACGT366400.043.29801612
ACGTCTG369150.042.84819815
CGTCTGA368950.042.8348416
ACACGTC370100.042.80725513
AGCACAC371600.042.8003810
GTCACAC362700.042.68003529
TCGGAAG376650.042.49623
GTATGCC361850.042.4250545
GAGCACA377150.042.271969
CACACTT364400.042.24004731
TCTGAAC373500.042.2106318
CGTATGC366350.041.8977944
TCACACT367500.041.8714930
CTGAACT380550.041.47549419
GTCTGAA417750.037.93873617
TCGTATG411550.037.48752643
AGAGCAC427350.037.4590348
ACTCCAG419350.037.42708623
CTCCAGT418500.037.39393224