FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l06n01_glk1_chx_pn_pd_01.35100000040761.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l06n01_glk1_chx_pn_pd_01.35100000040761.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29580427
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC10517823.5556687535308398TruSeq Adapter, Index 2 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA3409011.1524546281904584No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC2592190.8763193310225036No Hit
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC544910.18421302708037313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1158250.043.6525312
CGTCTGA1162950.043.5652416
ACGTCTG1164700.043.53068515
ACACGTC1163650.043.45073713
GTATGCC1161600.043.2244445
GCACACG1170450.043.22037511
TCTGAAC1175950.042.9879818
TCGGAAG1197850.042.9707683
AGCACAC1183100.042.8989810
CGTATGC1171650.042.87095644
ACCGATG1180200.042.62750632
GAGCACA1192200.042.6092769
CCGATGT1177400.042.60301233
CTCGTAT1182950.042.47855442
TATCTCG1180400.042.47686439
TAAGCCG368950.042.44412616
CTGAACT1190350.042.39220419
TCGTATG1188100.042.32850643
TCTCGTA1190800.042.19845641
GTCTGAA1203950.042.1003317