FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l06n01_glk1_chx_pn_md_02i.351000000408f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l06n01_glk1_chx_pn_md_02i.351000000408f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26434561
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC3475261.3146652974490478TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG383000.043.52387611
CACACGT383250.043.49010512
AGCACAC386350.043.34448210
ACACGTC384600.043.27015313
TCGGAAG391050.043.1393173
ACGTCTG384100.043.10170415
CGTCTGA384700.042.97599416
GAGCACA390450.042.900869
TCTGAAC385100.042.73858318
GTATGCC379450.042.7045245
CTGAACT388450.042.3348519
CGTATGC383450.042.27085544
CGATCAG383250.042.017533
CACGATC384300.041.990531
AGTCACG420150.038.73435228
GTCACGA418950.038.6895129
AAGAGCA454200.037.270777
ACTCCAG442550.037.0867723
TCACGAT437100.037.0829830
CTCCAGT443600.036.98649624
Produced by FastQC (version 0.11.2)