FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l06n01_glk1_chx_pn_md_02.351000000407a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l06n01_glk1_chx_pn_md_02.351000000407a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28066566
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC24259808.64366520649516TruSeq Adapter, Index 4 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA1422510.5068343594296502No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC1071950.3819312986134463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC2624600.044.25868213
CGTCTGA2621700.044.1902916
ACGTCTG2625900.044.16244515
TCGGAAG2655750.044.128093
CACACGT2635950.044.107812
GCACACG2641250.044.07605411
GATCGGA2670900.043.9258881
CGGAAGA2666000.043.902684
ATCGGAA2679000.043.787122
CACGTCT2658050.043.67568214
AGCACAC2672400.043.60439310
TCTGAAC2654650.043.5850118
GAGCACA2681100.043.5308729
AGAGCAC2687350.043.462288
ACCAATC2581550.043.3835336
ACTGACC2617950.043.38236232
GTATGCC2596850.043.35489345
GTCTGAA2672800.043.34543217
CTGAACT2671450.043.3215619
GACCAAT2593350.043.229535
Produced by FastQC (version 0.11.2)