FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l06n01_glk1_chx_mn_pd_03i.3510000004096f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l06n01_glk1_chx_mn_pd_03i.3510000004096f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26694473
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC1840220.6893636746453096TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT196750.043.29467412
ACTAGCT192300.043.10758232
CACTAGC192450.043.07036231
GCACACG197950.043.04350311
GTCACTA192600.043.03682329
ACACGTC198500.042.87648813
CGTCTGA197650.042.87634716
AGTCACT193950.042.85330228
AGCACAC199450.042.8100310
GTATGCC194750.042.8100145
ACGTCTG198800.042.7075415
TCTGAAC198400.042.6915918
TAGCTTA193700.042.67033834
GAGCACA200550.042.664979
GCTTATC193850.042.6373236
CGTATGC195950.042.6052444
TTATCTC195450.042.43791238
CTGAACT200100.042.4074319
TCGGAAG205250.041.962713
TAAGCCG18650.040.0477616