FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l06n01_glk1_chx_mn_pd_03.351000000407e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l06n01_glk1_chx_mn_pd_03.351000000407e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29362974
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC11396613.881286003250216TruSeq Adapter, Index 5 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA895500.3049759196735317No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC683650.2328272333722054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC1219900.044.04936213
CGTCTGA1216150.043.99757816
TCGGAAG1240000.043.71123
ACGTCTG1226250.043.70490315
GATCGGA1249200.043.459121
ATCGGAA1251100.043.3899422
CACACGT1240450.043.3616912
CTCGTAT1216750.043.35605642
CGTATGC1218750.043.32182344
TCTCGTA1217800.043.30744641
GCACACG1244000.043.2845311
CGGAAGA1252450.043.2240074
GTATGCC1222700.043.1873945
CACGTCT1245050.043.14072814
TCGTATG1226050.043.08406443
ATCTCGT1224900.043.0270340
TCTGAAC1259500.042.4314518
GTCACAC1255500.042.13855429
AGCACAC1279650.042.13316710
AGAGCAC1283900.042.048028
Produced by FastQC (version 0.11.2)