FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l06n01_glk1_chx_mn_md_04i.351000000409e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l06n01_glk1_chx_mn_md_04i.351000000409e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23750417
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC2086620.8785614164163938TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT239900.043.2826812
GCACACG240400.043.22945811
ACACGTC240100.043.19713613
ACGTCTG239350.043.18858715
GTATGCC234200.043.03309245
CGTCTGA240150.043.01661316
AGCACAC242300.043.0111910
TCTGAAC240000.042.97797418
CACGGCT234250.042.94785331
GAGCACA243500.042.8639039
GCTACAT233300.042.84214435
CATCTCG233550.042.82518439
CTGAACT241500.042.71962719
CGTATGC236050.042.6481844
CTACATC234400.042.56431236
TCGGAAG249450.042.1596953
AGTCACG275750.036.72912228
TAAGCCG9600.036.088316
ACGGCTA289150.035.00405532
TCACGGC289300.034.8610230
Produced by FastQC (version 0.11.2)