FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l06n01_glk1_chx_mn_md_04.3510000004082d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l06n01_glk1_chx_mn_md_04.3510000004082d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23255172
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC11379624.893371676631761TruSeq Adapter, Index 6 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA2860421.23001455332173No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC2202810.9472344474596877No Hit
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC446080.19181969499086055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC1218700.043.9085213
CGTCTGA1216050.043.86114516
CACACGT1222100.043.82086612
ACGTCTG1217800.043.809215
GCACACG1229750.043.597111
TCGGAAG1239800.043.5320783
GTATGCC1212400.043.5028645
CGTATGC1217200.043.32576844
AGTCACG1220350.043.1664128
CGCCAAT1214600.043.1447633
AGCACAC1245150.043.11390710
TCTGAAC1237650.043.05748718
TAAGCCG301400.043.0377516
TATCTCG1227600.042.9347139
CTCGTAT1228300.042.93240442
TCGTATG1231500.042.8628643
GAGCACA1253500.042.842869
TCTCGTA1230650.042.83012441
CGGAAGA1260050.042.770864
GATCGGA1265650.042.708791