FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l05n01_nlp7_chip_pchx_input_60.35100000040c29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l05n01_nlp7_chip_pchx_input_60.35100000040c29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12950061
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC1338531.0336090308763797TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG147750.043.29276711
ACGTCTG148950.042.99809315
CACACGT150200.042.631212
GTATGCC149350.042.46118545
CGTATGC149500.042.3734444
ACACGTC151800.042.17827613
CGTCTGA151850.042.147316
CACAGTG150150.041.9804133
GTCACAC152050.041.6097129
GAGCACA155500.041.164039
TCGGAAG155850.041.147123
AGCACAC155550.041.1363310
CAGTGAT155250.040.55772435
TCTCGTA157500.040.32111741
ACAGTGA156850.040.12965434
CTGAACT160450.039.97235519
ATCTCGT158650.039.9437740
CTCGTAT167250.038.0512642
ACTCCAG170000.037.63901523
GATCGGA172900.037.489381
Produced by FastQC (version 0.11.2)