FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l05n01_nlp7_chip_pchx_input_5.35100000040af2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l05n01_nlp7_chip_pchx_input_5.35100000040af2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19928820
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC2498051.2534861572335945TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG271800.043.1022411
ACGTCTG272950.042.83241315
GTATGCC272350.042.6047445
CGTATGC274600.042.28841844
ACACGTC277050.042.23335313
CACACGT277350.042.231312
CGTCTGA278450.041.9702216
CCGATGT276400.041.92381333
GTCACCG278450.041.68455529
ACCGATG278800.041.6396932
AGTCACC279900.041.4203628
TCGGAAG284150.041.3176773
AGCACAC284250.041.27770610
TCACCGA281550.041.21759430
GAGCACA286000.041.0487339
TCTCGTA285650.040.6760741
CTGAACT290000.040.34559619
ATCTCGT291100.039.91452840
CGATGTA292550.039.60904334
TATCTCG297000.039.09889239
Produced by FastQC (version 0.11.2)