FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l05n01_nlp7_chip_pchx_input_30.35100000040bca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l05n01_nlp7_chip_pchx_input_30.35100000040bca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12050237
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC1977411.6409718746610544TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG212050.043.533211
ACGTCTG212350.043.4878315
GTATGCC210750.043.2842745
ACACGTC213700.043.22506713
CACACGT213950.043.1676312
TCGGAAG214450.043.117963
CGTCTGA214400.043.04054316
CGTATGC212450.042.959144
GAGCACA215700.042.869569
AGCACAC216450.042.6898310
AGTCACT214650.042.56949228
TCTCGTA215600.042.42535841
CTGAACT218750.042.18535219
ATCTCGT216800.042.16977340
ACCAATC222450.040.95763836
ACTGACC230350.039.72109232
TCTGAAC233700.039.50531418
GATCGGA236050.039.4581761
CTCGTAT233450.039.33561742
AGAGCAC235650.039.2688878
Produced by FastQC (version 0.11.2)