FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l05n01_nlp7_chip_pchx_input_180.35100000040c86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l05n01_nlp7_chip_pchx_input_180.35100000040c86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15668124
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1844961.1775245077202605TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG198650.043.1969711
ACGTCTG200650.042.7498715
CACGCCA199600.042.63355331
CACACGT202600.042.39906312
ACACGTC202550.042.36280413
CGTATGC201350.042.14317344
GTATGCC201650.042.1139445
CGCCAAT203350.041.83957733
CGTCTGA206550.041.57230816
GAGCACA208600.041.1904539
AGCACAC209150.041.11440310
TCGGAAG210100.040.947583
TCTCGTA209850.040.45759641
GCCAATA211150.040.3258334
CTGAACT214300.040.0695119
AGTCACG213850.039.89776228
ATCTCGT213850.039.7639740
TCACGCC217450.039.1544330
ACGCCAA220000.038.67482432
TATCTCG221200.038.6360539
Produced by FastQC (version 0.11.2)