FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l05n01_nlp7_chip_pchx_input_10.35100000040b6d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l05n01_nlp7_chip_pchx_input_10.35100000040b6d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13691014
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC2369591.7307629661323845TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG249600.043.7730611
CACACGT250250.043.65904212
ACGTCTG250000.043.6415315
ACACGTC251050.043.53386713
CGTCTGA251200.043.48678216
TCGGAAG251750.043.4612163
GTATGCC249800.043.41531445
GAGCACA253000.043.2025879
CGTATGC251000.043.19878844
AGCACAC253000.043.19369510
CACTTAG251450.043.1531231
TAGGCAT250250.043.1394835
CATCTCG251300.043.03084639
AGTCACT252550.042.97407528
TCTCGTA252450.042.9595841
CTGAACT254900.042.82938819
ATCTCGT253350.042.7892140
TCACTTA255000.042.53470630
ACTTAGG268800.040.38490332
GATCGGA275600.039.929721
Produced by FastQC (version 0.11.2)