FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l05n01_nlp7_chip_pchx_input_0.35100000040a95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l05n01_nlp7_chip_pchx_input_0.35100000040a95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17835922
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC3412841.913464299742957TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG360600.043.21936811
GTATGCC359900.043.02919445
ACGTCTG363750.042.86443315
CGTATGC362450.042.7326744
CACACGT365200.042.6871912
ACACGTC366000.042.60933713
CGTCTGA367300.042.44401616
GAGCACA371600.041.946069
TCGGAAG372850.041.9388963
TCACGAT369700.041.74265735
AGCACAC374250.041.7031510
CACGATC370650.041.65387336
TCTCGTA373750.041.47078341
CTGAACT377250.041.24130219
ATCTCGT377400.041.05181540
CTCGTAT382500.040.55150642
TCGTATG383200.040.4656943
ACTCCAG385850.040.23960523
CATCACG385550.040.16114433
AGAGCAC388950.040.1154488
Produced by FastQC (version 0.11.2)