FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l05n01_nlp7_chip_pchx_60.351000000409cc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l05n01_nlp7_chip_pchx_60.351000000409cc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16676985
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC1644860.9863053783402695TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG182450.042.3105211
CGTATGC181450.041.42088744
ACGTCTG185900.041.36114515
GTATGCC182150.041.3605145
CACGGCT182900.041.2622531
CACACGT189500.040.6764612
ACACGTC190150.040.47497613
AGTCACG188250.040.25697728
CGTCTGA191250.040.2276416
ACGGCTA191150.039.66180432
GAGCACA195450.039.5423629
AGCACAC195150.039.51091410
CGGCTAC191850.039.46923433
TCGGAAG195900.039.4548343
GTCACGG191750.039.42826529
TCTCGTA193150.039.08653341
TCACGGC194350.038.87763630
GCTACAT194800.038.68617235
CATCTCG195000.038.6118839
CTCGTAT196150.038.500242
Produced by FastQC (version 0.11.2)