FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l05n01_nlp7_chip_pchx_5.3510000004087d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l05n01_nlp7_chip_pchx_5.3510000004087d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13895754
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC881320.6342369043090429TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG99350.041.4200911
ACGTCTG101750.040.56993515
CGTATGC100450.040.5127144
GTATGCC101800.039.95335845
ACACGTC104350.039.5832113
CACACGT104700.039.38912212
CGTCTGA105900.039.02256816
GTCACAC106750.038.0558529
AGCACAC108850.037.88778710
GAGCACA108950.037.853019
TCGGAAG109900.037.5420153
TCTCGTA109900.037.0905441
CTGAACT114700.036.0094619
ATCTCGT114700.035.6560440
CACACTT116850.034.86277831
CTCGTAT118550.034.63091742
ACTCCAG118900.034.62793723
TCGTATG119300.034.4320643
TCACACT118350.034.36386530
AGAGCAC120500.034.280798
Produced by FastQC (version 0.11.2)