FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l05n01_nlp7_chip_pchx_30.35100000040946.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l05n01_nlp7_chip_pchx_30.35100000040946.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23038268
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC2411791.0468625506049327TruSeq Adapter, Index 10 (100% over 51bp)
ACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTG251800.10929641065031452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG265700.042.440611
ACGTCTG269350.041.83488515
ACACGTC271350.041.53690313
GTATGCC267650.041.51225345
CACACGT271900.041.4725812
CGTATGC268550.041.38988544
CGTCTGA272700.041.28796416
CACTAGC272600.040.72101231
TCGGAAG278700.040.5742073
AGCACAC278700.040.5416810
GAGCACA280500.040.281529
TCTCGTA277350.040.11718841
ACTAGCT280650.039.5293232
AGTCACT283150.039.354828
CTGAACT288600.039.0603719
GCTTATC284900.038.86455536
ATCTCGT286250.038.82272340
TAGCTTA288550.038.41191534
TATCTCG293850.038.0023639
AGAGCAC298600.037.892558
Produced by FastQC (version 0.11.2)