FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l05n01_nlp7_chip_pchx_180.35100000040a38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l05n01_nlp7_chip_pchx_180.35100000040a38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13257332
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC1354961.0220457630539839TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG149250.042.43457411
CGTATGC150650.041.4974244
ACGTCTG152450.041.47965215
GTATGCC152300.041.09215545
CACACGT154450.040.97629512
ACACGTC154750.040.8653113
CGTCTGA157300.040.2293216
AGTCACC155900.040.09183528
GAGCACA159450.039.6918149
AGCACAC160850.039.3743210
TCGGAAG162000.039.236683
TCTCGTA160700.038.95821441
CTGAACT162850.038.8450619
CTCGTAT163150.038.44212742
ATCTCGT164350.037.98349840
AGAGCAC167650.037.7504358
TCGTATG166400.037.6777843
ACTCCAG167350.037.64375723
GATCGGA170850.037.283561
ATCGGAA172850.036.9039652
Produced by FastQC (version 0.11.2)