FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l05n01_nlp7_chip_pchx_10.351000000408e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l05n01_nlp7_chip_pchx_10.351000000408e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13446668
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC1331340.9900891432732629TruSeq Adapter, Index 9 (100% over 51bp)
ACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTG222580.1655279954855731No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG147850.042.48188415
GCACACG147900.042.41172811
CGTATGC147500.042.17089544
CACACGT148950.042.12769712
ACACGTC149350.042.02743513
GTATGCC148800.041.78734645
CGTCTGA150400.041.7765716
TCGGAAG153250.041.1268773
CGATCAG151050.041.01611333
GAGCACA153950.040.876549
TCTCGTA152050.040.86457441
AGCACAC154100.040.85135710
TCACGAT152300.040.6676230
CACGATC152300.040.59373531
CTGAACT155100.040.42358819
ATCTCGT154150.040.29327840
AGATCTC160950.038.63284738
AGTCACG160300.038.6240128
GTCACGA161950.038.216629
AGAGCAC169600.037.1974838