FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l05n01_nlp7_chip_pchx_0.35100000040803.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l05n01_nlp7_chip_pchx_0.35100000040803.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12210773
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC714740.5853355885004168TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC82700.040.5306544
GCACACG84350.040.4100211
ACGTCTG85250.039.97815
GTATGCC84800.039.57999845
CACACGT87750.038.94651812
ACACGTC88200.038.6437113
CGTCTGA88800.038.43044316
TCGGAAG92000.037.0668373
AGCACAC92750.036.89578610
AGTCACC90950.036.6738428
TCTCGTA92150.036.39863641
CATCTCG93150.036.08033439
CTCGTAT93250.036.01751742
GAGCACA95350.035.842529
TCGTATG94800.035.45235443
ATCTCGT96700.034.7557740
CCAGATC96300.034.7370433
CTGAACT98600.034.61092819
GATCGGA99750.034.49691
CAGTCAC98600.034.14802627
Produced by FastQC (version 0.11.2)