FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l04n01_nlp7_chip_mchx_input_60.35100000040d5f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l04n01_nlp7_chip_mchx_input_60.35100000040d5f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9479478
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC3410323.5975820609531453TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG370700.043.94096811
TCGGAAG371600.043.935333
GTATGCC365800.043.86184745
ACGTCTG370150.043.85691515
CACACGT370900.043.85684612
ACACGTC371250.043.76938213
CACGGCT368400.043.70369331
CGTCTGA371500.043.6975416
CGTATGC368600.043.58946244
GAGCACA373950.043.5650949
GCTACAT368750.043.41921235
CATCTCG369250.043.40329439
AGCACAC375200.043.39596610
CTGAACT374450.043.3900219
TCTCGTA370650.043.34860241
CTACATC369050.043.3107736
ATCTCGT371850.043.22081840
GATCGGA384350.042.695651
AGTCACG381450.042.37394728
ATCGGAA386600.042.3179632
Produced by FastQC (version 0.11.2)