FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l04n01_nlp7_chip_mchx_input_5.35100000040c79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l04n01_nlp7_chip_mchx_input_5.35100000040c79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14660527
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC1661661.1334244669376483TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG184150.043.15161511
ACGTCTG186800.042.5250915
CGTATGC186150.042.27490644
GTATGCC185750.042.2691945
ACACGTC188900.042.0285213
CACACGT189350.041.93092312
GTCACAC191100.041.25322329
CGTCTGA193150.041.1503316
TCGGAAG194400.040.886573
GAGCACA195300.040.7569969
AGCACAC195900.040.55177710
TCTCGTA196050.040.18617241
CTGAACT198450.039.973219
ATCTCGT199400.039.54474340
CACACTT201050.039.22252331
CTCGTAT202700.039.01206642
TCGTATG204050.038.797843
TCACACT204050.038.712330
TCTGAAC207250.038.34103418
ACTCCAG206750.038.2966123
Produced by FastQC (version 0.11.2)