FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l04n01_nlp7_chip_mchx_input_30.35100000040d0e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l04n01_nlp7_chip_mchx_input_30.35100000040d0e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13631342
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC1690271.2399879630340138TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG187050.043.01224511
GTATGCC187550.042.49952745
CGTATGC187850.042.4910744
ACGTCTG189250.042.43810315
ACACGTC191200.041.98175413
CACACGT192000.041.8566212
CGTCTGA192000.041.84198416
CACTAGC191900.041.5396631
TCGGAAG194650.041.3752753
AGCACAC195950.041.07012610
GAGCACA196650.040.9811339
AGTCACT197350.040.49558628
TCTCGTA197800.040.29690641
ACTAGCT198300.040.13385432
CTGAACT200150.040.0833219
ATCTCGT199850.039.8610440
GCTTATC201300.039.4617236
TATCTCG203350.039.21921539
CTCGTAT204950.039.03378342
TAGCTTA203400.039.03217334
Produced by FastQC (version 0.11.2)