FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l04n01_nlp7_chip_mchx_input_180.35100000040d92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l04n01_nlp7_chip_mchx_input_180.35100000040d92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12017475
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC1449211.2059188806300825TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG159900.043.33579611
ACGTCTG162250.042.63755415
CACACGT162750.042.5632812
GTATGCC161600.042.43334645
CGTATGC162300.042.3470144
ACACGTC163500.042.2840613
CGTCTGA165400.041.82553516
AGTCACC165950.041.56466328
TCGGAAG167350.041.4712373
AGCACAC168000.041.25978510
GAGCACA169050.041.016829
TCTCGTA168350.040.78543541
CTGAACT172050.040.31419419
ATCTCGT171150.040.10504540
TGTAATC173200.039.53927236
CTCGTAT175950.039.16439442
TCGTATG177000.038.99545743
GATCGGA180800.038.62171
ACTCCAG180950.038.3837923
AGAGCAC181200.038.291358
Produced by FastQC (version 0.11.2)