FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l04n01_nlp7_chip_mchx_input_10.35100000040cc9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l04n01_nlp7_chip_mchx_input_10.35100000040cc9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11561328
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC2247321.9438251384270042TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG236100.043.7963411
CACACGT237250.043.59353612
ACGTCTG237150.043.54249215
GTATGCC235600.043.389945
ACACGTC238350.043.3515913
CGTCTGA238600.043.2684516
TCGGAAG240050.043.1781773
GAGCACA240200.043.1330769
CGATCAG236850.043.0942333
CACGATC237300.043.06551731
TCACGAT238300.042.99868430
CGTATGC238200.042.98221644
AGCACAC241400.042.937310
TCTCGTA239400.042.74797441
ATCTCGT240100.042.63271340
CTGAACT242300.042.5347419
AGATCTC243750.041.88356438
AGTCACG253550.040.50124728
AGAGCAC256650.040.3859948
GTCACGA254150.040.37906629