FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l04n01_nlp7_chip_mchx_input_0.35100000040c0f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l04n01_nlp7_chip_mchx_input_0.35100000040c0f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12484719
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC2021161.6189070815290276TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG215450.043.21142611
ACGTCTG217100.042.87991715
CACACGT217850.042.74604812
GTATGCC215900.042.68078245
CGTATGC216050.042.6303244
ACACGTC219350.042.46058713
CGTCTGA219850.042.3640216
TCGGAAG221800.042.0958333
GAGCACA222750.041.8356939
AGTCACC221050.041.69058628
CATCTCG222400.041.56470539
AGCACAC225400.041.36380410
TCTCGTA223350.041.317441
CCAGATC223900.041.10521733
CTGAACT226550.041.0528819
ATCTCGT225350.040.98065640
ACCAGAT229850.040.11689432
CTCGTAT230600.040.0476642
TCGTATG232200.039.7717143
ACTCCAG234300.039.55631623
Produced by FastQC (version 0.11.2)