FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l04n01_nlp7_chip_mchx_60.35100000040b2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l04n01_nlp7_chip_mchx_60.35100000040b2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21813761
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC2143050.982430310848276TruSeq Adapter, Index 5 (100% over 51bp)
ACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTG261810.12002056866764058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG242100.042.4780411
ACGTCTG243900.042.0514415
GTATGCC242450.041.8206245
CGTATGC243200.041.80246444
ACACGTC247800.041.42593413
CACACGT248550.041.3576112
CGTCTGA248950.041.20745516
CACAGTG247250.040.90846333
GTCACAC249650.040.65709729
AGCACAC255350.040.32673610
TCGGAAG256250.040.2550473
GAGCACA257350.040.030829
TCTCGTA255400.039.8320641
ATCTCGT261400.038.91778640
CTGAACT264900.038.71842619
CAGTGAT261600.038.71603435
ACAGTGA263400.038.40875634
AGAGCAC274000.037.68048
ACTCCAG272900.037.50483723
CTCGTAT272850.037.44952442
Produced by FastQC (version 0.11.2)