FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l04n01_nlp7_chip_mchx_5.351000000409d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l04n01_nlp7_chip_mchx_5.351000000409d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11993230
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC725890.6052497950927315TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG82750.041.08130611
CGTATGC82750.040.2906744
ACGTCTG85200.039.84500515
GTATGCC83850.039.62795645
CACACGT87000.039.04876312
ACACGTC87650.038.80825813
CCGATGT87050.038.68792333
ACCGATG87150.038.5734932
GTCACCG88700.037.90575429
CGTCTGA89950.037.76591516
AGTCACC89500.037.71780428
GAGCACA91150.037.3941579
TCGGAAG90800.037.3249473
AGCACAC91550.037.18162510
TCACCGA91000.036.9229730
TCTCGTA91350.036.81772241
CTCGTAT92950.036.2807742
CGATGTA92950.036.28061734
TCGTATG92900.036.15543
ACTCCAG96350.035.23927723
Produced by FastQC (version 0.11.2)