FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l04n01_nlp7_chip_mchx_30.35100000040abe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l04n01_nlp7_chip_mchx_30.35100000040abe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8432624
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC590860.7006834408838815TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG65400.041.830911
CGTATGC66350.040.55236444
ACGTCTG67700.040.44107415
GTATGCC66700.040.3398145
ACACGTC68250.040.04924813
CACACGT68550.039.97456712
CGTCTGA70100.038.99231716
AGCACAC71150.038.5135710
GAGCACA71200.038.360139
TCGGAAG72550.037.6231273
AGTCACT73300.037.1829528
TCTCGTA73100.036.83856641
ACTGACC74100.036.28693832
CTGAACT75800.036.1793219
CTCGTAT74700.036.16998742
ATCTCGT74800.036.031440
TCGTATG75050.036.00130543
ACTCCAG75700.035.84565723
CAGTCAC76900.035.41299427
AGAGCAC77550.035.3931668
Produced by FastQC (version 0.11.2)