FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l04n01_nlp7_chip_mchx_180.35100000040ba1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l04n01_nlp7_chip_mchx_180.35100000040ba1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20125874
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1232490.6123907960469195TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG140850.041.22643711
ACGTCTG144850.039.94659815
CACGCCA143350.039.8759731
CGTATGC144550.039.67154744
GTATGCC144450.039.6526845
CACACGT148400.039.1897412
ACACGTC150100.038.62434413
CGCCAAT149400.038.2179433
CGTCTGA152550.038.01877216
TCACGCC153400.037.30769330
AGCACAC156800.037.1906310
AGTCACG154950.037.13783328
GAGCACA157400.037.0774549
TCGGAAG159100.036.717953
GCCAATA156700.036.3944634
GTCACGC159200.036.07571829
TCTCGTA160150.035.89156741
CTGAACT164100.035.41237619
ACGCCAA163650.035.01884532
CTCGTAT165850.034.75298342
Produced by FastQC (version 0.11.2)