FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l04n01_nlp7_chip_mchx_10.35100000040a45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l04n01_nlp7_chip_mchx_10.35100000040a45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11212366
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC954480.8512743875824246TruSeq Adapter, Index 3 (100% over 51bp)
ACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTG131690.11745067900922963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG107050.042.6853211
ACGTCTG108900.041.83342415
ACACGTC109200.041.6978913
CGTATGC109200.041.4300744
GTATGCC109400.041.39583245
CACACGT110550.041.3746112
CGTCTGA110550.041.22939316
AGCACAC112500.040.63745510
GAGCACA112900.040.5333339
TAGGCAT110650.040.4398635
CACTTAG111000.040.42738331
TCGGAAG113250.040.3769763
TCTCGTA113400.039.9156441
AGTCACT114050.039.6226828
CATCTCG114250.039.5202239
CTGAACT116900.038.9523719
ATCTCGT116900.038.66282740
TCACTTA119950.037.63622730
AGAGCAC124850.036.7257738
ACTCCAG124800.036.50889623
Produced by FastQC (version 0.11.2)