FastQCFastQC Report
Tue 26 Jan 2016
C7KY3ACXX_l04n01_nlp7_chip_mchx_0.35100000040953.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KY3ACXX_l04n01_nlp7_chip_mchx_0.35100000040953.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14520645
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC1172590.8075329987063247TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG128650.041.93576411
ACGTCTG130650.041.11948415
GTATGCC131200.040.69018645
CGTATGC131850.040.57448244
ACACGTC134500.040.0762713
CGTCTGA135350.039.7414816
CACACGT136100.039.62397812
AGCACAC139350.038.87717410
GAGCACA139700.038.699259
TCGGAAG140800.038.3888663
TCACGAT138750.038.32959735
TCTCGTA141050.037.88015741
CACGATC140650.037.77982336
CTCGTAT145050.036.92860842
CTGAACT147200.036.54331619
TCGTATG147600.036.47351543
ATCTCGT147050.036.4110540
ACTCCAG147550.036.40015823
CATCACG146400.036.37281433
AGAGCAC150450.036.0387768
Produced by FastQC (version 0.11.2)